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KMID : 1377020190160030237
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Tissue Engineering and Regenerative Medicine
2019 Volume.16 No. 3 p.237 ~ p.251
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Preparation and Characterization of Nanocomposite Scaffolds (Collagen/β-TCP/SrO) for Bone Tissue Engineering
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Goodarzi Hamid
Hashemi-Najafabadi Sameereh
Baheiraei Nafiseh
Bagheri Fatemeh
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Abstract
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Background: Nowadays, production of nanocomposite scaffolds based on natural biopolymer, bioceramic, and metal ions is a growing field of research due to the potential for bone tissue engineering applications.
Methods: In this study, a nanocomposite scaffold for bone tissue engineering was successfully prepared using collagen (COL), beta-tricalcium phosphate (β-TCP) and strontium oxide (SrO). A composition of β-TCP (4.9 g) was prepared by doping with SrO (0.05 g). Biocompatible porous nanocomposite scaffolds were prepared by freeze-drying in different formulations [COL, COL/β-TCP (1:2 w/w), and COL/β-TCP-Sr (1:2 w/w)] to be used as a provisional matrix or scaffold for bone tissue engineering. The nanoparticles were characterized by X-ray diffraction, Fourier transforms infrared spectroscopy and energy dispersive spectroscopy. Moreover, the prepared scaffolds were characterized by physicochemical properties, such as porosity, swelling ratio, biodegradation, mechanical properties, and biomineralization.
Results: All the scaffolds had a microporous structure with high porosity (~?95?99%) and appropriate pore size (100?200 μm). COL/β-TCP-Sr scaffolds had the compressive modulus (213.44?±?0.47 kPa) higher than that of COL/β-TCP (33.14?±?1.77 kPa). In vitro cytocompatibility, cell attachment and alkaline phosphatase (ALP) activity studies performed using rat bone marrow mesenchymal stem cells. Addition of β-TCP-Sr to collagen scaffolds increased ALP activity by 1.33?1.79 and 2.92?4.57 folds after 7 and 14 days of culture, respectively.
Conclusion: In summary, it was found that the incorporation of Sr into the collagen-β-TCP scaffolds has a great potential for bone tissue engineering applications.
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KEYWORD
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Collagen, β-TCP, SrO, Freeze drying, Bone tissue engineering
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